recombinant phage meaning in Chinese
重组噬菌体
Examples
- Tg1 and 15 separate colonies were assayed by elisa . the recombinant phages which were rescued from colony 12 showed the highest affinity to immobilized hcg . the scfv gene was inserted into the reconstructed pet - 21a and expressed in
用elisa方法进行检测，从结合力最强的克隆中分离单链抗体基因，插入经改造的高效表达载体pet - 21a中，转化大肠杆菌，诱导表达。 - The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e . coli bl21 . furthermore , library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm
首先用牛精子免疫雌性四周龄balb c小鼠，从其脾脏组织分离总rna ，应用重组噬菌体抗体库技术，构建了一个针对牛精子的噬菌体抗体文库。 - The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。 - Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen , the positive dones possessing apparent inhibitory effect were singled out for later use
X阳性克到驶知烘扳原kbbjg ）浊对阳性克隆进行限制陇卧赐析（ uwi和hedlll ）鉴定三用竞争elisatoljmg7重组噬菌体抗体性克隆对mg7单扶与其相应抗原结合忙的喇率，从中j ） ed出对mg7单抗与期眩抗原结合有抑余j效应的克隆用于进一涉研究。 - Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii , the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa
3 mg _ 7重组噬菌体抗体库的淘筛用m13ko7辅助噬菌体感染转化菌，以挽救出噬菌体形式的抗体库；用高表达mg _ 7抗原的胃癌细胞系kato对抗体库进行三轮淘筛；用elisa筛检呈现有mg _ 7scfv的噬菌体单克隆。